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Construction, imaging and analysis of FRET-based tension sensors in living cells

Due to an increased appreciation for the importance of mechanical stimuli in many biological contexts, an interest in measuring the forces experienced by specific proteins in living cells has recently emerged. The development and use of Forster resonance energy transfer (FRET)-based molecular tensio...

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Bibliographic Details
Published in:Methods Cell Biol
Main Authors: LaCroix, Andrew S., Rothenberg, Katheryn E., Berginski, Matthew E., Urs, Aarti N., Hoffman, Brenton D.
Format: Artigo
Language:Inglês
Published: 2015
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4511473/
https://ncbi.nlm.nih.gov/pubmed/25640429
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mcb.2014.10.033
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