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Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells

Segmentation is a periodic and sequential morphogenetic process in vertebrates. This rhythmic formation of blocks of tissue called somites along the body axis is evidence of a genetic oscillator patterning the developing embryo. In zebrafish, the intracellular clock driving segmentation is comprised...

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Détails bibliographiques
Publié dans:J Vis Exp
Auteurs principaux: Webb, Alexis B., Soroldoni, Daniele, Oswald, Annelie, Schindelin, Johannes, Oates, Andrew C.
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2014
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4511270/
https://ncbi.nlm.nih.gov/pubmed/25078855
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50307
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