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Silencing of Essential Genes within a Highly Coordinated Operon in Escherichia coli

Essential bacterial genes located within operons are particularly challenging to study independently because of coordinated gene expression and the nonviability of knockout mutants. Essentiality scores for many operon genes remain uncertain. Antisense RNA (asRNA) silencing or in-frame gene disruptio...

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Bibliografske podrobnosti
izdano v:Appl Environ Microbiol
Main Authors: Goh, Shan, Hohmeier, Angela, Stone, Timothy C., Offord, Victoria, Sarabia, Francisco, Garcia-Ruiz, Cristina, Good, Liam
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4510190/
https://ncbi.nlm.nih.gov/pubmed/26070674
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01444-15
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