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Silencing of Essential Genes within a Highly Coordinated Operon in Escherichia coli

Essential bacterial genes located within operons are particularly challenging to study independently because of coordinated gene expression and the nonviability of knockout mutants. Essentiality scores for many operon genes remain uncertain. Antisense RNA (asRNA) silencing or in-frame gene disruptio...

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Dades bibliogràfiques
Publicat a:Appl Environ Microbiol
Autors principals: Goh, Shan, Hohmeier, Angela, Stone, Timothy C., Offord, Victoria, Sarabia, Francisco, Garcia-Ruiz, Cristina, Good, Liam
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4510190/
https://ncbi.nlm.nih.gov/pubmed/26070674
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01444-15
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