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Increased efficiency of evolved group I intron spliceozymes by decreased side product formation
The group I intron ribozyme from Tetrahymena was recently reengineered into a trans-splicing variant that is able to remove 100-nt introns from pre-mRNA, analogous to the spliceosome. These spliceozymes were improved in this study by 10 rounds of evolution in Escherichia coli cells. One clone with i...
Αποθηκεύτηκε σε:
| Τόπος έκδοσης: | RNA |
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| Κύριοι συγγραφείς: | , |
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
Cold Spring Harbor Laboratory Press
2015
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| Θέματα: | |
| Διαθέσιμο Online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4509937/ https://ncbi.nlm.nih.gov/pubmed/26106216 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.051888.115 |
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