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DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.

Computer simulations of the evolution of linear sequences have demonstrated the importance of recombination of blocks of sequence rather than point mutagenesis alone. Repeated cycles of point mutagenesis, recombination, and selection should allow in vitro molecular evolution of complex sequences, su...

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Autor principal: Stemmer, W P
Format: Artigo
Idioma:Inglês
Publicat: 1994
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC45099/
https://ncbi.nlm.nih.gov/pubmed/7938023
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