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Formation and reshuffling of disulfide bonds in bovine serum albumin demonstrated using tandem mass spectrometry with collision-induced and electron-transfer dissociation

Thermolysin hydrolyzates of freshly isolated, extensively stored (6 years, 6 °C, dry) and heated (60 min, 90 °C, in excess water) bovine serum albumin (BSA) samples were analyzed with liquid chromatography (LC) electrospray ionization (ESI) tandem mass spectrometry (MS/MS) using alternating electron...

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Veröffentlicht in:Sci Rep
Hauptverfasser: Rombouts, Ine, Lagrain, Bert, Scherf, Katharina A., Koehler, Peter, Delcour, Jan A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group 2015
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4507448/
https://ncbi.nlm.nih.gov/pubmed/26193081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep12210
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