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Correction of depth-induced spherical aberration for deep observation using two-photon excitation fluorescence microscopy with spatial light modulator

We demonstrate fluorescence imaging with high fluorescence intensity and depth resolution in which depth-induced spherical aberration (SA) caused by refractive-index mismatch between the medium and biological sample is corrected. To reduce the impact of SA, we incorporate a spatial light modulator i...

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Detalhes bibliográficos
Publicado no:Biomed Opt Express
Main Authors: Matsumoto, Naoya, Inoue, Takashi, Matsumoto, Akiyuki, Okazaki, Shigetoshi
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4505711/
https://ncbi.nlm.nih.gov/pubmed/26203383
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.6.002575
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