TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR

The advent of fluorescence-based quantitative real-time PCR (qPCR) has revolutionized the quantification of gene expression analysis in many fields, including life sciences, agriculture, forensic science, molecular diagnostics, and medicine. While SYBR Green-based qPCR is the most commonly-used plat...

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Udgivet i:PLoS One
Main Authors: Zhang, Qian, Wang, Jing, Deng, Fang, Yan, Zhengjian, Xia, Yinglin, Wang, Zhongliang, Ye, Jixing, Deng, Youlin, Zhang, Zhonglin, Qiao, Min, Li, Ruifang, Denduluri, Sahitya K., Wei, Qiang, Zhao, Lianggong, Lu, Shun, Wang, Xin, Tang, Shengli, Liu, Hao, Luu, Hue H., Haydon, Rex C., He, Tong-Chuan, Jiang, Li
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2015
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4501803/
https://ncbi.nlm.nih.gov/pubmed/26172450
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0132666
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