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A novel immunogold cryoelectron microscopic approach to investigate the structure of the intracellular and extracellular forms of vaccinia virus.

We introduce a novel approach for combining immunogold labelling with cryoelectron microscopy of thin vitrified specimens. The method takes advantage of the observation that particles in suspension are concentrated at the air-water interface and remain there during the subsequent immunogold labellin...

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Detalhes bibliográficos
Publicado no:EMBO J
Main Authors: Roos, N, Cyrklaff, M, Cudmore, S, Blasco, R, Krijnse-Locker, J, Griffiths, G
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 1996
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC450163/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8665841/
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