A novel immunogold cryoelectron microscopic approach to investigate the structure of the intracellular and extracellular forms of vaccinia virus.

We introduce a novel approach for combining immunogold labelling with cryoelectron microscopy of thin vitrified specimens. The method takes advantage of the observation that particles in suspension are concentrated at the air-water interface and remain there during the subsequent immunogold labellin...

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Bibliographic Details
Main Authors: Roos, N, Cyrklaff, M, Cudmore, S, Blasco, R, Krijnse-Locker, J, Griffiths, G
Format: Artigo
Language:Inglês
Published: 1996
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC450163/
https://ncbi.nlm.nih.gov/pubmed/8665841
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