Nicked-site substrates for a serine recombinase reveal enzyme–DNA communications and an essential tethering role of covalent enzyme–DNA linkages

To analyse the mechanism and kinetics of DNA strand cleavages catalysed by the serine recombinase Tn3 resolvase, we made modified recombination sites with a single-strand nick in one of the two DNA strands. Resolvase acting on these sites cleaves the intact strand very rapidly, giving an abnormal ha...

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Vydáno v:Nucleic Acids Res
Hlavní autoři: Olorunniji, Femi J., McPherson, Arlene L., Pavlou, Hania J., McIlwraith, Michael J., Brazier, John A., Cosstick, Richard, Stark, W. Marshall
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2015
Témata:
Nucleic Acid Enzymes
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4499144/
https://ncbi.nlm.nih.gov/pubmed/25990737
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv521
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