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Trace fluorescent labeling for protein crystallization
Fluorescence can be a powerful tool to aid in the crystallization of proteins. In the trace-labeling approach, the protein is covalently derivatized with a high-quantum-yield visible-wavelength fluorescent probe. The final probe concentration typically labels ≤0.20% of the protein molecules, which h...
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| Published in: | Acta Crystallogr F Struct Biol Commun |
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| Main Authors: | , , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
International Union of Crystallography
2015
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4498700/ https://ncbi.nlm.nih.gov/pubmed/26144224 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X15008626 |
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