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Trace fluorescent labeling for protein crystallization

Fluorescence can be a powerful tool to aid in the crystallization of proteins. In the trace-labeling approach, the protein is covalently derivatized with a high-quantum-yield visible-wavelength fluorescent probe. The final probe concentration typically labels ≤0.20% of the protein molecules, which h...

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Библиографические подробности
Опубликовано в: :Acta Crystallogr F Struct Biol Commun
Главные авторы: Pusey, Marc, Barcena, Jorge, Morris, Michelle, Singhal, Anuj, Yuan, Qunying, Ng, Joseph
Формат: Artigo
Язык:Inglês
Опубликовано: International Union of Crystallography 2015
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4498700/
https://ncbi.nlm.nih.gov/pubmed/26144224
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X15008626
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