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Tryptophan Scanning Reveals Dense Packing of Connexin Transmembrane Domains in Gap Junction Channels Composed of Connexin32

Tryptophan was substituted for residues in all four transmembrane domains of connexin32. Function was assayed using dual cell two-electrode voltage clamp after expression in Xenopus oocytes. Tryptophan substitution was poorly tolerated in all domains, with the greatest impact in TM1 and TM4. For ins...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Brennan, Matthew J., Karcz, Jennifer, Vaughn, Nicholas R., Woolwine-Cunningham, Yvonne, DePriest, Adam D., Escalona, Yerko, Perez-Acle, Tomas, Skerrett, I. Martha
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4498046/
https://ncbi.nlm.nih.gov/pubmed/25969535
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M115.650747
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