Methyl labeling and TROSY NMR spectroscopy of proteins expressed in the eukaryote Pichia pastoris

(13)C Methyl TROSY NMR spectroscopy has emerged as a powerful method for studying the dynamics of large systems such as macromolecular assemblies and membrane proteins. Specific (13)C labeling of aliphatic methyl groups and perdeuteration has been limited primarily to proteins expressed in E. coli,...

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Udgivet i:J Biomol NMR
Main Authors: Clark, Lindsay, Zahm, Jacob A., Ali, Rustam, Kukula, Maciej, Bian, Liangqiao, Patrie, Steven M., Gardner, Kevin H., Rosen, Michael K., Rosenbaum, Daniel M.
Format: Artigo
Sprog:Inglês
Udgivet: 2015
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4496254/
https://ncbi.nlm.nih.gov/pubmed/26025061
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-015-9939-2
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