Digital microfluidic immunocytochemistry in single cells

We report a new technique called Digital microfluidic Immunocytochemistry in Single Cells (DISC). DISC automates protocols for cell culture, stimulation and immunocytochemistry, enabling the interrogation of protein phosphorylation on pulsing with stimulus for as little as 3 s. DISC was used to prob...

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Bibliografiske detaljer
Udgivet i:Nat Commun
Main Authors: Ng, Alphonsus H. C., Chamberlain, M. Dean, Situ, Haozhong, Lee, Victor, Wheeler, Aaron R.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2015
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4491823/
https://ncbi.nlm.nih.gov/pubmed/26104298
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms8513
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