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Digital microfluidic immunocytochemistry in single cells

We report a new technique called Digital microfluidic Immunocytochemistry in Single Cells (DISC). DISC automates protocols for cell culture, stimulation and immunocytochemistry, enabling the interrogation of protein phosphorylation on pulsing with stimulus for as little as 3 s. DISC was used to prob...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Ng, Alphonsus H. C., Chamberlain, M. Dean, Situ, Haozhong, Lee, Victor, Wheeler, Aaron R.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4491823/
https://ncbi.nlm.nih.gov/pubmed/26104298
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms8513
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