Enzymatic completion of mammalian lagging-strand DNA replication.

Using purified proteins from calf and a synthetic substrate, we have reconstituted the enzymatic reactions required for mammalian Okazaki fragment processing in vitro. The required reactions are removal of initiator RNA, synthesis from an upstream fragment to generate a nick, and then ligation. With...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Turchi, J J, Huang, L, Murante, R S, Kim, Y, Bambara, R A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1994
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC44905/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7524089/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.21.9803
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