Enzymatic completion of mammalian lagging-strand DNA replication.

Using purified proteins from calf and a synthetic substrate, we have reconstituted the enzymatic reactions required for mammalian Okazaki fragment processing in vitro. The required reactions are removal of initiator RNA, synthesis from an upstream fragment to generate a nick, and then ligation. With...

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Bibliografiska uppgifter
Huvudupphovsmän: Turchi, J J, Huang, L, Murante, R S, Kim, Y, Bambara, R A
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1994
Ämnen:
Research Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC44905/
https://ncbi.nlm.nih.gov/pubmed/7524089
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