Expression and Characterization of the RKOD DNA Polymerase in Pichia pastoris

The present study assessed high-level expression of the KOD DNA polymerase in Pichia pastoris. Thermococcus kodakaraensis KOD1 is a DNA polymerase that is widely used in PCR. The DNA coding sequence of KOD was optimized based on the codon usage bias of P. pastoris and synthesized by overlapping PCR,...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Wang, Fei, Li, Shuntang, Zhao, Hui, Bian, Lu, Chen, Liang, Zhang, Zhen, Zhong, Xing, Ma, Lixin, Yu, Xiaolan
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4489709/
https://ncbi.nlm.nih.gov/pubmed/26134129
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0131757
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