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Rapid method for the isolation of mammalian sperm DNA

The unique DNA packaging of spermatozoa renders them resistant to DNA isolation techniques used for somatic cells, requiring alternative methods that are slow and labor intensive. Here we present a rapid method for isolating high-quality sperm DNA. Isolated human sperm cells were homogenized with 0....

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Détails bibliographiques
Publié dans:Biotechniques
Auteurs principaux: Wu, Haotian, de Gannes, Matthew K., Luchetti, Gianna, Pilsner, J. Richard
Format: Artigo
Langue:Inglês
Publié: 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4486329/
https://ncbi.nlm.nih.gov/pubmed/26054765
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114280
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