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Molecular Orientation of Factor VIIIa on the Phospholipid Membrane Surface Determined by Fluorescence Resonance Energy Transfer
Factor VIIIa (FVIIIa) binds to phospholipid membranes during formation of the FXase complex. Free thiols of Cys residues of isolated FVIIIa A1 and A2 subunits and the A3 domain of A3C1C2 subunit were labeled with PyMPO or fluorescein (fluorescence donors). Double mutants in the A3 domain (Cys2000Ser...
Enregistré dans:
| Publié dans: | Biochem J |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2013
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4471871/ https://ncbi.nlm.nih.gov/pubmed/23521092 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20130025 |
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