An optimized fluorescent probe for visualizing glutamate neurotransmission

We describe an intensity-based glutamate-sensing fluorescent reporter (“iGluSnFR”) with signal-to-noise ratio and kinetics appropriate for in vivo imaging. We engineered iGluSnFR in vitro to maximize its fluorescence change, and validated its utility for visualizing glutamate release by neurons and...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Marvin, Jonathan S., Borghuis, Bart G., Tian, Lin, Cichon, Joseph, Harnett, Mark T., Akerboom, Jasper, Gordus, Andrew, Renninger, Sabine L., Chen, Tsai-Wen, Bargmann, Cornelia I., Orger, Michael B., Schreiter, Eric R., Demb, Jonathan B., Gan, Wen-Biao, Hires, S. Andrew, Looger, Loren L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4469972/
https://ncbi.nlm.nih.gov/pubmed/23314171
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2333
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