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Effective heritable gene knockdown in zebrafish using synthetic microRNAs

Although zebrafish is used to model human diseases through mutational and morpholino-based knockdown approaches, there are currently no robust transgenic knockdown tools. Here we investigate the knockdown efficiency of three synthetic miRNA-expressing backbones and show that these constructs can dow...

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Bibliografiske detaljer
Udgivet i:Nat Commun
Main Authors: Giacomotto, Jean, Rinkwitz, Silke, Becker, Thomas S.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Pub. Group 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4468906/
https://ncbi.nlm.nih.gov/pubmed/26051838
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms8378
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