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Deletion of Genes Encoding Arginase Improves Use of “Heavy” Isotope-Labeled Arginine for Mass Spectrometry in Fission Yeast

The use of “heavy” isotope-labeled arginine for stable isotope labeling by amino acids in cell culture (SILAC) mass spectrometry in the fission yeast Schizosaccharomyces pombe is hindered by the fact that under normal conditions, arginine is extensively catabolized in vivo, resulting in the appearan...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Borek, Weronika E., Zou, Juan, Rappsilber, Juri, Sawin, Kenneth E.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4468061/
https://ncbi.nlm.nih.gov/pubmed/26075619
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0129548
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