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Tracking surface glycans on live cancer cells with single molecule sensitivity()
Using a combination of metabolically labeled glycans, bioorthogonal Cu(I)-catalyzed azide-alkyne cycloaddition and controlled bleaching of fluorescent probes conjugated to azide or alkyne tagged glycans, we achieve a sufficiently low spatial density of dye labeled glycans enabling dynamic single-mol...
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| Publicado no: | Angew Chem Int Ed Engl |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4465920/ https://ncbi.nlm.nih.gov/pubmed/25515330 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201407976 |
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