Highly Sensitive Assay for Detection of Enterovirus in Clinical Specimens by Reverse Transcription-PCR with an Armored RNA Internal Control

The objective of the present study was the development of a diagnostic reverse transcription (RT)-PCR for the specific detection of enterovirus (EV) RNA in clinical specimens controlled by an internal control (IC) RNA. The IC RNA contains the same primer binding sites as EV RNA but has a different p...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Beld, Marcel, Minnaar, René, Weel, Jan, Sol, Cees, Damen, Marjolein, van der Avoort, Harry, Wertheim-van Dillen, Pauline, Breda, Alex van, Boom, René
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2004
Schlagworte:
Virology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC446274/
https://ncbi.nlm.nih.gov/pubmed/15243060
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.7.3059-3064.2004
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