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Sensing pH via p-Cyanophenylalanine Fluorescence: Application to Determine Peptide pK(a) and Membrane-Penetration Kinetics
We expand the spectroscopic utility of a well-known infrared and fluorescence probe, p-cyanophenylalanine, by showing that it can also serve as a pH sensor. This new application is based on the notion that the fluorescence quantum yield of this unnatural amino acid, when placed at or near the N-term...
Gardado en:
| Publicado en: | Anal Biochem |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2015
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4461448/ https://ncbi.nlm.nih.gov/pubmed/25935260 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.04.026 |
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