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Directed Evolution of RecA Variants with Enhanced Capacity for Conjugational Recombination

The recombination activity of Escherichia coli (E. coli) RecA protein reflects an evolutionary balance between the positive and potentially deleterious effects of recombination. We have perturbed that balance, generating RecA variants exhibiting improved recombination functionality via random mutage...

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Veröffentlicht in:PLoS Genet
Hauptverfasser: Kim, Taejin, Chitteni-Pattu, Sindhu, Cox, Benjamin L., Wood, Elizabeth A., Sandler, Steven J., Cox, Michael M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2015
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4457935/
https://ncbi.nlm.nih.gov/pubmed/26047498
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pgen.1005278
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