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An Approach to Heterologous Expression of Membrane Proteins. The Case of Bacteriorhodopsin

Heterologous overexpression of functional membrane proteins is a major bottleneck of structural biology. Bacteriorhodopsin from Halobium salinarum (bR) is a striking example of the difficulties in membrane protein overexpression. We suggest a general approach with a finite number of steps which allo...

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Publicat a:PLoS One
Autors principals: Bratanov, Dmitry, Balandin, Taras, Round, Ekaterina, Shevchenko, Vitaly, Gushchin, Ivan, Polovinkin, Vitaly, Borshchevskiy, Valentin, Gordeliy, Valentin
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4457421/
https://ncbi.nlm.nih.gov/pubmed/26046789
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0128390
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