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An Approach to Heterologous Expression of Membrane Proteins. The Case of Bacteriorhodopsin
Heterologous overexpression of functional membrane proteins is a major bottleneck of structural biology. Bacteriorhodopsin from Halobium salinarum (bR) is a striking example of the difficulties in membrane protein overexpression. We suggest a general approach with a finite number of steps which allo...
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| Publicat a: | PLoS One |
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| Autors principals: | , , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Public Library of Science
2015
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4457421/ https://ncbi.nlm.nih.gov/pubmed/26046789 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0128390 |
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