Catch me if you can: a biotinylated proteoliposome affinity assay for the investigation of assembly of the MexA-MexB-OprM efflux pump from Pseudomonas aeruginosa

Efflux pumps are membrane transporters that actively extrude various substrates, leading to multidrug resistance (MDR). In this study, we have designed a new test that allows investigating the assembly of the MexA-MexB-OprM efflux pump from the Gram negative bacteria Pseudomonas aeruginosa. The meth...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Ntsogo Enguéné, Véronique Yvette, Verchère, Alice, Phan, Gilles, Broutin, Isabelle, Picard, Martin
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2015
Assuntos:
Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4451422/
https://ncbi.nlm.nih.gov/pubmed/26082762
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2015.00541
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