siRNA-mediated gene silencing of MexB from the MexA-MexB-OprM efflux pump in Pseudomonas aeruginosa

To gain insights into the effect of MexB gene under the short interfering RNA (siRNA), we synthesized 21 bp siRNA duplexes against the MexB gene. RT-PCR was performed to determine whether the siRNA inhibited the expression of MexB mRNA. Changes in antibiotic susceptibility in response to siRNA were...

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Detalhes bibliográficos
Main Authors: Gong, Feng-Yun, Zhang, Ding-Yu, Zhang, Jiang-Guo, Wang, Li-Li, Zhan, Wei-Li, Qi, Jun-Ying, Song, Jian-Xin
Formato: Artigo
Idioma:Inglês
Publicado em: Korean Society for Biochemistry and Molecular Biology 2014
Assuntos:
Research Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4163889/
https://ncbi.nlm.nih.gov/pubmed/24219865
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5483/BMBRep.2014.47.4.040
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