siRNA-mediated gene silencing of MexB from the MexA-MexB-OprM efflux pump in Pseudomonas aeruginosa

To gain insights into the effect of MexB gene under the short interfering RNA (siRNA), we synthesized 21 bp siRNA duplexes against the MexB gene. RT-PCR was performed to determine whether the siRNA inhibited the expression of MexB mRNA. Changes in antibiotic susceptibility in response to siRNA were...

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Autors principals: Gong, Feng-Yun, Zhang, Ding-Yu, Zhang, Jiang-Guo, Wang, Li-Li, Zhan, Wei-Li, Qi, Jun-Ying, Song, Jian-Xin
Format: Artigo
Idioma:Inglês
Publicat: Korean Society for Biochemistry and Molecular Biology 2014
Matèries:
Research Articles
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4163889/
https://ncbi.nlm.nih.gov/pubmed/24219865
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5483/BMBRep.2014.47.4.040
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