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Random insertion and gene disruption via transposon mutagenesis of Ureaplasma parvum using a mini-transposon plasmid
While transposon mutagenesis has been successfully used for Mycoplasma spp. to disrupt and determine non-essential genes, previous attempts with Ureaplasma spp. have been unsuccessful. Using a polyethylene glycol-transformation enhancing protocol, we were able to transform three separate serovars of...
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| Publicado no: | Int J Med Microbiol |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4450083/ https://ncbi.nlm.nih.gov/pubmed/25444567 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ijmm.2014.09.003 |
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