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The effect of immunological status, in-vitro treatment and culture time on expression of eleven candidate reference genes in bovine blood mononuclear cells
BACKGROUND: Technical feasibility of RNA quantification by real time RT-PCR has led to enormous utilization of this method. However, real time PCR results need to be normalized due to the high sensitivity of the method and also to eliminate technical variation. Normalization against a reference gene...
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| Veröffentlicht in: | BMC Immunol |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2015
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4449592/ https://ncbi.nlm.nih.gov/pubmed/26025301 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12865-015-0099-7 |
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