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The effect of immunological status, in-vitro treatment and culture time on expression of eleven candidate reference genes in bovine blood mononuclear cells

BACKGROUND: Technical feasibility of RNA quantification by real time RT-PCR has led to enormous utilization of this method. However, real time PCR results need to be normalized due to the high sensitivity of the method and also to eliminate technical variation. Normalization against a reference gene...

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Publicado en:BMC Immunol
Autores principales: Emam, Mehdi, Thompson-Crispi, Kathleen, Mallard, Bonnie
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2015
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4449592/
https://ncbi.nlm.nih.gov/pubmed/26025301
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12865-015-0099-7
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