Accurate Estimation of Viral Abundance by Epifluorescence Microscopy

Virus enumeration by epifluorescence microscopy (EFM) is routinely done on preserved, refrigerated samples. Concerns about obtaining accurate and reproducible estimates led us to examine procedures for counting viruses by EFM. Our results indicate that aldehyde fixation results in rapid decreases in...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Wen, Kevin, Ortmann, Alice C., Suttle, Curtis A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2004
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC444834/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15240256/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.7.3862-3867.2004
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