Accurate Estimation of Viral Abundance by Epifluorescence Microscopy

Virus enumeration by epifluorescence microscopy (EFM) is routinely done on preserved, refrigerated samples. Concerns about obtaining accurate and reproducible estimates led us to examine procedures for counting viruses by EFM. Our results indicate that aldehyde fixation results in rapid decreases in...

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Publicado en:Appl Environ Microbiol
Main Authors: Wen, Kevin, Ortmann, Alice C., Suttle, Curtis A.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 2004
Assuntos:
Methods
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC444834/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15240256/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.7.3862-3867.2004
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