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Use of the Riboflavin Synthase Gene (ribC) as a Model for Development of an Essential Gene Disruption and Complementation System for Haemophilus influenzae

We have developed a system for rapid and reliable assessment of gene essentiality in Haemophilus influenzae Rd strain KW20. We constructed two “suicide” complementation vectors (pASK5 and pASK6) containing 5′ and 3′ regions of the nonessential ompP1 gene flanking a multiple cloning site and a select...

詳細記述

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書誌詳細
主要な著者: Saeed-Kothe, Amna, Yang, Wei, Mills, Scott D.
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology 2004
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC444764/
https://ncbi.nlm.nih.gov/pubmed/15240293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.7.4136-4143.2004
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