Use of the Riboflavin Synthase Gene (ribC) as a Model for Development of an Essential Gene Disruption and Complementation System for Haemophilus influenzae

We have developed a system for rapid and reliable assessment of gene essentiality in Haemophilus influenzae Rd strain KW20. We constructed two “suicide” complementation vectors (pASK5 and pASK6) containing 5′ and 3′ regions of the nonessential ompP1 gene flanking a multiple cloning site and a select...

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Bibliographic Details
Main Authors: Saeed-Kothe, Amna, Yang, Wei, Mills, Scott D.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2004
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC444764/
https://ncbi.nlm.nih.gov/pubmed/15240293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.7.4136-4143.2004
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