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Use of the Riboflavin Synthase Gene (ribC) as a Model for Development of an Essential Gene Disruption and Complementation System for Haemophilus influenzae

We have developed a system for rapid and reliable assessment of gene essentiality in Haemophilus influenzae Rd strain KW20. We constructed two “suicide” complementation vectors (pASK5 and pASK6) containing 5′ and 3′ regions of the nonessential ompP1 gene flanking a multiple cloning site and a select...

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Bibliografiset tiedot
Päätekijät: Saeed-Kothe, Amna, Yang, Wei, Mills, Scott D.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: American Society for Microbiology 2004
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC444764/
https://ncbi.nlm.nih.gov/pubmed/15240293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.7.4136-4143.2004
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