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Development of Enhanced Capacity Affinity Microcolumns by using a Hybrid of Protein Cross-linking/Modification and Immobilization

A hybrid method was examined for increasing the binding capacity and activity of protein-based affinity columns by using a combination of protein cross-linking/modification and covalent immobilization. Various applications of this approach in the study of drug-protein interactions and in use with af...

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Detalhes bibliográficos
Publicado no:J Chromatogr A
Main Authors: Zheng, Xiwei, Podariu, Maria, Bi, Cong, Hage, David S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4447555/
https://ncbi.nlm.nih.gov/pubmed/25981291
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chroma.2015.04.051
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