FIB/SEM technology and high-throughput 3D reconstruction of dendritic spines and synapses in GFP-labeled adult-generated neurons

The fine analysis of synaptic contacts is usually performed using transmission electron microscopy (TEM) and its combination with neuronal labeling techniques. However, the complex 3D architecture of neuronal samples calls for their reconstruction from serial sections. Here we show that focused ion...

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Detalhes bibliográficos
Publicado no:Front Neuroanat
Main Authors: Bosch, Carles, Martínez, Albert, Masachs, Nuria, Teixeira, Cátia M., Fernaud, Isabel, Ulloa, Fausto, Pérez-Martínez, Esther, Lois, Carlos, Comella, Joan X., DeFelipe, Javier, Merchán-Pérez, Angel, Soriano, Eduardo
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2015
Assuntos:
Neuroscience
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4440362/
https://ncbi.nlm.nih.gov/pubmed/26052271
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fnana.2015.00060
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