FIB/SEM technology and high-throughput 3D reconstruction of dendritic spines and synapses in GFP-labeled adult-generated neurons

The fine analysis of synaptic contacts is usually performed using transmission electron microscopy (TEM) and its combination with neuronal labeling techniques. However, the complex 3D architecture of neuronal samples calls for their reconstruction from serial sections. Here we show that focused ion...

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Détails bibliographiques
Publié dans:Front Neuroanat
Auteurs principaux: Bosch, Carles, Martínez, Albert, Masachs, Nuria, Teixeira, Cátia M., Fernaud, Isabel, Ulloa, Fausto, Pérez-Martínez, Esther, Lois, Carlos, Comella, Joan X., DeFelipe, Javier, Merchán-Pérez, Angel, Soriano, Eduardo
Format: Artigo
Langue:Inglês
Publié: Frontiers Media S.A. 2015
Sujets:
Neuroscience
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4440362/
https://ncbi.nlm.nih.gov/pubmed/26052271
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fnana.2015.00060
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