Expression and purification of a single-chain Type IV restriction enzyme Eco94GmrSD and determination of its substrate preference

The first reported Type IV restriction endonuclease (REase) GmrSD consists of GmrS and GmrD subunits. In most bacteria, however, the gmrS and gmrD genes are fused together to encode a single-chain protein. The fused coding sequence for ECSTEC94C_1402 from E. coli strain STEC_94C was expressed in T7...

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Bibliographic Details
Published in:Sci Rep
Main Authors: He, Xinyi, Hull, Victoria, Thomas, Julie A., Fu, Xiaoqing, Gidwani, Sonal, Gupta, Yogesh K., Black, Lindsay W., Xu, Shuang-yong
Format: Artigo
Language:Inglês
Published: Nature Publishing Group 2015
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4437046/
https://ncbi.nlm.nih.gov/pubmed/25988532
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep09747
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