Receptor-binding domain of SARS-Cov spike protein: Soluble expression in E.coli, purification and functional characterization

AIM: To find a soluble and functional recombinant receptor-binding domain of severe acute respiratory syndrome-associated coronavirus (SARS-Cov), and to analyze its receptor binding ability. METHODS: Three fusion tags (glutathione S-transferase, GST; thioredoxin, Trx; maltose-binding protein, MBP),...

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Bibliographic Details
Published in:World J Gastroenterol
Main Authors: Chen, Jing, Miao, Lin, Li, Jia-Ming, Li, Yan-Ying, Zhu, Qing-Yu, Zhou, Chang-Lin, Fang, Hong-Qing, Chen, Hui-Peng
Format: Artigo
Language:Inglês
Published: Baishideng Publishing Group Inc 2005
Subjects:
Basic Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4436633/
https://ncbi.nlm.nih.gov/pubmed/16273643
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v11.i39.6159
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