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Mapping Homing Endonuclease Cleavage Sites Using In Vitro Generated Protein

Mapping the precise position of endonucleolytic cleavage sites is a fundamental experimental technique used to describe the function of a homing endonuclease. However, these proteins are often recalcitrant to cloning and over-expression in biological systems because of toxicity induced by spurious D...

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Bibliografiska uppgifter
I publikationen:Methods Mol Biol
Huvudupphovsmän: Bonocora, Richard P., Belfort, Marlene
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2014
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4435548/
https://ncbi.nlm.nih.gov/pubmed/24510259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-968-0_4
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