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Mapping Homing Endonuclease Cleavage Sites Using In Vitro Generated Protein
Mapping the precise position of endonucleolytic cleavage sites is a fundamental experimental technique used to describe the function of a homing endonuclease. However, these proteins are often recalcitrant to cloning and over-expression in biological systems because of toxicity induced by spurious D...
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| Publicado no: | Methods Mol Biol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4435548/ https://ncbi.nlm.nih.gov/pubmed/24510259 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-968-0_4 |
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