A GFP-based assay for rapid screening of compounds affecting ARE-dependent mRNA turnover

A reporter transcript containing the green fluorescent protein (GFP) gene upstream of the destabilizing 3′-untranslated region (3′-UTR) of the murine IL-3 gene was inserted in mouse PB-3c-15 mast cells. The GFP–IL-3 transcript was inherently unstable due to the presence of an adenosine–uridine (AU)-...

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Detalles Bibliográficos

Publicado en:	Nucleic Acids Res
Principais autores:	Benjamin, Don, Colombi, Marco, Moroni, Christoph
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 2004
Assuntos:	NAR Methods Online
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC443554/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15247322/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh086
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