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A GFP-based assay for rapid screening of compounds affecting ARE-dependent mRNA turnover

A reporter transcript containing the green fluorescent protein (GFP) gene upstream of the destabilizing 3′-untranslated region (3′-UTR) of the murine IL-3 gene was inserted in mouse PB-3c-15 mast cells. The GFP–IL-3 transcript was inherently unstable due to the presence of an adenosine–uridine (AU)-...

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Bibliografski detalji
Glavni autori: Benjamin, Don, Colombi, Marco, Moroni, Christoph
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2004
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC443554/
https://ncbi.nlm.nih.gov/pubmed/15247322
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh086
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