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A GFP-based assay for rapid screening of compounds affecting ARE-dependent mRNA turnover
A reporter transcript containing the green fluorescent protein (GFP) gene upstream of the destabilizing 3′-untranslated region (3′-UTR) of the murine IL-3 gene was inserted in mouse PB-3c-15 mast cells. The GFP–IL-3 transcript was inherently unstable due to the presence of an adenosine–uridine (AU)-...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
2004
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC443554/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15247322/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh086 |
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