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Identification of pork in meat products using real-time loop-mediated isothermal amplification

In this study, a one-step, real-time, loop-mediated isothermal amplification (RealAmp) assay was developed, for the highly specific detection of pork DNA. For the assay, the mtDNA of cytochrome b (cytb) gene was amplified at 63 °C using SYBR Green I for 45 min with a Real-Time Polymerase Chain React...

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Detalhes bibliográficos
Publicado no:Biotechnol Biotechnol Equip
Main Authors: Yang, Lixia, Fu, Shujun, Peng, Xinkai, Li, Le, Song, Taoping, Li, Lin
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4433938/
https://ncbi.nlm.nih.gov/pubmed/26019573
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/13102818.2014.963789
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