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Expression of heterologous sigma factors enables functional screening of metagenomic and heterologous genomic libraries
A key limitation in using heterologous genomic or metagenomic libraries in functional genomics and genome engineering is the low expression of heterologous genes in screening hosts, such as Escherichia coli. To overcome this limitation, here we generate E. coli strains capable of recognizing heterol...
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| Publicado no: | Nat Commun |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Pub. Group
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4432631/ https://ncbi.nlm.nih.gov/pubmed/25944046 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms8045 |
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